GLYTUNES Publications


“The lipooligosaccharide of the gut symbiont Akkermansia muciniphila exhibits a remarkable structure and TLR signaling capacity”
P. Garcia-Vello, H. Tytgat, J. Elzinga, M. Van Hul, H. Plovier, M. Tiemblo-Martín, P. Cani, S. Nicolardi, M. Fragai, C. De Castro, F. Di Lorenzo, A. Silipo, A. Molinaro & W. M. de Vos
Nat. Commun., 2024, 15, e202400479

https://www.nature.com/articles/s41467-024-52683-x


“Structure and properties of the exopolysaccharide isolated from Flavobacterium sp. Root935”
M. Tiemblo-Martìn, V. Pistorio, P. Saake, L. Mahdi, M. Asunción Campanero-Rhodes, R. Di Girolamo, C. di Carluccio, R. Marchetti, A. Molinaro, D. Solís, A. Zuccaro, A. Silipo
Carbohydr. Polym., 2024, 343, 122433

https://www.sciencedirect.com/science/article/pii/S0144861724006593


“The human gut Bacteroides eggerthii expresses a new galactofuranose-containing lipooligosaccharide with weak immunostimulatory properties”
M. Tiemblo-Martín, M. Coccimiglio, E. Andretta, L. De Simone Carone, A. Bell, T. Gerpe-Amor, C. Di Carluccio, A. Molinaro, Y. van Kooyk, N. Juge, F. Chiodo, F. Di Lorenzo, A. Silipo
Carbohydr. Polym., 2025, 348, 122833

https://www.sciencedirect.com/science/article/abs/pii/S0144861724010592


“Parametric Analysis of Donor Activation for Glycosylation Reactions”
M-H. Lin, Y-T. Kuo, J. Danglad-Flores, E. T. Sletten, P. H. Seeberger
Chem. Eur. J., 2024, 30, e202400479

https://chemistry-europe.onlinelibrary.wiley.com/doi/full/10.1002/chem.202400479


“Quantifying Siglec-sialylated ligand interactions: a versatile 19F-T2 CPMG filtered competitive NMR displacement assay”
U. Atxabal, A. Fernández, M. Jesús Moure, K. Sobczak, C. Nycholat, V. Almeida-Marrero, I. Oyenarte, J. C. Paulson, A. de la Escosura, T. Torres, N. C. Reichardt, J. Jiménez-Barbero, J. Ereño-Orbea
Chem. Sci., 2024, 15, 10612-10624

https://pubs.rsc.org/en/content/articlelanding/2024/sc/d4sc01723d


“Mammalian cell-based production of glycans, glycopeptides and glycomodules”
T. Jaroentomeechai, R. Karlsson, F. Goerdeler, F. K. Yik Teoh, M. Nørregaard Grønset, D. de Wit, Y-H Chen, S. Furukawa, V. Psomiadou, R. Hurtado-Guerrero, E. E. Vidal-Calvo, A. Salanti, T. J Boltje, L. J van den Bos, C. Wunder, L. Johannes, K. T Schjoldager, H. J Joshi, R. L Miller, H. Clausen, S. Y Vakhrushev, Y. Narimatsu
Nat Commun., 2024, 15, 9668

https://www.nature.com/articles/s41467-024-53738-9


“Insights into Siglec-7 binding to gangliosides: NMR protein assignment and the impact of ligand flexibility”
C. Di Carluccio, L. Padilla-Cortés, M. Tiemblo-Martìn, G. R. Gheorghita, R. Oliva, L. Cerofolini, A.A. Masi, C. Abreu, H.K. Tseng, A. Molinaro, P. Del Vecchio, O. Vaněk, C.C. Lin, R. Marchetti, M. Fragai, A. Silipo
Advanced Science, 2025, Online Version of Record before inclusion in an issue 2415782

https://advanced.onlinelibrary.wiley.com/doi/10.1002/advs.202415782?af=R


“Molecular Basis of Siglec-7 Recognition by Neisseria meningitidis Serogroup Y CPS: Implications for Immune Evasion”
C. Di Carluccio, T. Gerpe Amor, M. P. Lenza, A. A. Masia, C. Abreu, V. Longo, F. Albano, F. Nieto-Fabregat, P. Salvatore, G. Falco, D. Santana-Medero, M. Fragai, Y. van Kooyk, A. Molinaro, Y. Valdes-Balbin, O. Vaněk, V. Verez-Bencomo, R. Marchetti, F. Chiodo, A. Silipo
JACS Au, 2025, accepted

https://pubs.acs.org/doi/10.1021/jacsau.5c00214


“The conformational features of Fusobacterium nucleatum subsp. Polymorphum 10953 LPS O-antigen mediate its interaction with Siglec-7 and unveil a novel binding epitope”
C. Di Carluccio, F. Nieto-Fabregat, L. Cerofolini, C. Abreu, L. Padilla-Cortés, G. R. Gheorghita, A. A. Masi, L. Buono, M. Gumah Adam Ali, D. Lamprinaki, A. Molinaro, N. Juge, G. Smaldone, O. Vaněk, M. Fragai, R. Marchetti, A. Silipo
Submitted to Adv. Sci, 2025


“Solid-state NMR – a complementary technique for protein framework characterization”
L. Cerofolini, K. O. Ramberg, L. C. Padilla, P. Antonik, E. Ravera,C. Luchinat, M. Fragai, P. B. Crowley.
Chem. Commun., 2023, 59, 776-779

https://pubs.rsc.org/en/content/articlelanding/2023/cc/d2cc05725e


“Combining Solid-State NMR with Structural and Biophysical Techniques to Design Challenging Protein-Drug Conjugates”
L. Cerofolini, K. Vasa, E. Bianconi, M. Salobehaj, G. Cappelli, A. Bonciani, G. Licciardi, A. Pérez-Ràfols, L. Padilla-Cortés, S. Antonacci, D. Rizzo, E. Ravera, C. Viglianisi, V. Calderone, G. Parigi, C. Luchinat, A. Macchiarulo, S. Menichetti, M. Fragai.
Angew Chem Int Ed Engl., 2023, 62(31):e202303202

https://onlinelibrary.wiley.com/doi/10.1002/anie.202303202


“Structural insights into Siglec-15 reveal glycosylation dependency for its interaction with T cells through integrin CD11b”
M. P. Lenza, L. Egia-Mendikute, A. Antoñana-Vildosola, C. O. Soares, H. Coelho, F. Corzana, A. Bosch, P. Manisha, J. Imanol Quintana, I. Oyenarte, L. Unione, M. J. Moure, M. Azkargorta, U. Atxabal, K. Sobczak, F. Elortza, J. D. Sutherland, R. Barrio, F. Marcelo, J. Jiménez-Barbero, A. Palazon & J. Ereño-Orbea.
Nat Commun., 2023, 14, 1, 3496

https://www.nature.com/articles/s41467-023-39119-8


“UV light-induced spatial loss of sialic acid capping using a photoactivatable sialyltransferase inhibitor”
S. J. Moons, D. L.A.H. Hornikx, M. K. M. Aasted, J. F.A. Pijnenborg, M. Calzari, P. B. White, Y. Narimatsu, H. Clausen, H. H. Wandall, T. J. Boltje, and C. Büll.
RSC Chem Biol., 2023, 4, 7, 506-511

https://pubs.rsc.org/en/content/articlelanding/2023/cb/d3cb00006k


“Engineering the sialome of mammalian cells with sialic acid mimetics”
D. L.A.H. Hornikx, E. A. Visser, V. Psomiadou, C. Büll, and T. J. Boltje.
STAR Protoc., 2023, 4, 3, 102330

https://www.sciencedirect.com/science/article/pii/S2666166723002976?via%3Dihub


“A photoaffinity glycan-labeling approach to investigate immunoglobulin glycan-binding partners”
M. D Holborough-Kerkvliet, G. Mucignato, S. J Moons, V. Psomiadou, R. S R Konada, N. J Pedowitz, M. R Pratt, T. Kissel, C. A M Koeleman, R. T N Tjokrodirijo, P. A van Veelen, T. Huizinga, K. A J van Schie, M. Wuhrer, J. J Kohler, Kimberly M Bonger, T. J Boltje, R. E M Toes.
Glycobiology, 2023, 33, 9, 732–744

https://academic.oup.com/glycob/article/33/9/732/7232176


“The sialic acid-Siglec immune checkpoint: an opportunity to enhance immune responses and therapy effectiveness in melanoma”
M. Coccimiglio, F. Chiodo, Y. van Kooyk.
Br J Dermatol., 2024 Jan, 10:ljad517

https://academic.oup.com/bjd/advance-article/doi/10.1093/bjd/ljad517/7513897


“Molecular Insights into O-Linked Sialoglycans Recognition by the Siglec-Like SLBR-N (SLBRUB10712) of Streptococcus gordonii”
C. Di Carluccio, L. Cerofolini, M. Moreira, F. Rosu, L. Padilla-Cortés, G. R. Gheorghita, Z. Xu, A. Santra, H. Yu, S. Yokoyama, T. E. Gray, C. D. St. Laurent, Y. Manabe, X. Chen, K. Fukase, M. S. Macauley, A. Molinaro, T. Li, B. A. Bensing, R. Marchetti, V. Gabelica, M. Fragai, and A. Silipo.
ACS Cent. Sci., 2024, 10, 2, 447–459

https://pubs.acs.org/doi/10.1021/acscentsci.3c01598


“Site-Selective Functionalized PD-1 Mutant for a Modular Immunological Activity against Cancer Cells”
S. Fallarini, L. Cerofolini, M. Salobehaj, D. Rizzo, G. R. Gheorghita, G. Licciardi, D. E. Capialbi, V. Zullo, A. Sodini, C. Nativi, and M. Fragai.
Biomacromolecules, 2023, 24, 11, 5428–5437

https://pubs.acs.org/doi/10.1021/acs.biomac.3c00893


“Deciphering the Chemical Language of the Immunomodulatory Properties of Veillonella parvula Lipopolysaccharide”
M. D. Pither, E. Andretta, G. Rocca, F. Balzarini, A. Matamoros-Recio, R. Colicchio, P. Salvatore, Y. van Kooyk, A. Silipo, F. Granucci, S. Martin-Santamaria, F. Chiodo, A. Molinaro, F. Di Lorenzo.
Angew Chem Int Ed Engl., 2024 Feb, 23:e202401541

https://onlinelibrary.wiley.com/doi/10.1002/anie.202401541


“Sialic acids in infection and their potential use in detection and protection against pathogens”
S. Dedola, S. Ahmadipour, P. de Andrade, A. N. Baker, A. N. Boshra, S. Chessa, M. I. Gibson, P. J. Hernando, I. M. Ivanova, J. E. Lloyd, M. J. Marín, Alexandra J. Munro-Clark, G. Pergolizzi, S.-J. Richards, I. Ttofi, B. A. Wagstaff and R. A. Field.
RSC Chem. Biol., 2024, 5, 167-188

https://pubs.rsc.org/en/content/articlelanding/2024/cb/d3cb00155e